V8 Protease, also known as Endoproteinase Glu C, is a
staphylococcal serine proteinase
Sequencing Grade V8 Protease
Full Name:Sequencing grade recombinant V8 protease;Staphylococcus aureus
Synonyms:V8 protease;Endoproteinase Glu-C;Endoproteinase Asp-C
Storage temperature: 2-8°C
Source: Recombinant V8 protease is a genetically engineered protein
expressed in E.coli
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine
proteinase that selectively cleaves peptide bonds C-terminal to
glutamic acid residues. It also cleaves at aspartic acid
residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are
Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 -
macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
Approx.5U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the
substrate(approx.500U/mg at 37℃ with casein as the substrate).
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant V8 protease should be stored under 2-8°C in sealed
container.It is stable within 6 months after dissolved with1mM HCl
and 50mM HAC at -20°C or below. It is stable within 2 months after
dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can
be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C
12h. It is no activity loss after 10 times repeated freezing and
Stability of transport: The product is stable by blue ice insulation transport.