Endoproteinase Glu-C V8 is an extracellular bacterial serine
protease of the glutamyl endopeptidase I family
Full Name:Endoproteinase Glu-C,from Staphylococcus aureus strain V8,V8
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine
proteinase that selectively cleaves peptide bonds C-terminal to
glutamic acid residues. It also cleaves at aspartic acid
residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are
Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 -
macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
Approx.5U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the
substrate(approx.500U/mg at 37℃ with casein as the substrate).
Cutting condition:PH 8.0~8.5.
V8 protease should be stored under -20℃ in sealed container.
Protein structure and sequence analysis.