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ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Contact Person:Mr zhao

ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

Brand Name : yaxin
Model Number : RCPB01
Certification : ISO 9001
Place of Origin : CHINA
MOQ : 1G
Price : $1950
Payment Terms : T/T
Supply Ability : 1000G
Delivery Time : 3-10 DAY
Packaging Details : 1G,10G,50G
Source : E. Coli
Purified by : HPLC
Format : White or white-like or yellowish lyophilized
Specific activity : ≥170 units/mg pro.
Contaminant activity : Less than 10 ppm of recombinant trypsin.
cas : 9025-24-5
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Stability of RCPB solution under -20 and repeated freezing-thawing


ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u


Recombinant Carboxypeptidase B


REAGENT


1) Reagent A. 25 mM Tris-HCl Buffer with 100 mM Sodium Chloride, pH 7.65 at 25°C±0.5°C. (Prepare 100 ml in deionized water using Tris Hydrochloride, and Sodium Chloride. Adjust to pH 7.65 at 25°C with 1 M NaOH or 1M HCl.)

2) 1.0 mM Hippuryl-L-Arginine Solution (Hippuryl-L-Arg)

Weight 16.7 mg Hippuryl-L-Arginine (Sigma Prod. No. H-2508), dissolve in 50 ml Reagent A to obtain 1.0 mM (Hippuryl-L-Arg). PREPARE FRESH.).

3) RCPB Solution

Three batches RCPB lyophilized powder (RC110919, RC110106 and RC110304), prepare 10 mg/ml with reagent A, respectively, and separated into 30 tubes for each batch, stored under -20℃.


METHOD:


Long term stability of RCPB solution: The initial activity was measure just after preparation and taken as 100%. The activity was measure every 2 months, 3 parallel samples for each batch each time, take average, calculate residual rate of activity compared with initial one and then draw a stability curve.

Stability of RCPB solution during 10 cycles repeated freezing and thawing:The initial activity was measure just after preparation and taken as 100%. The activity of 3 samples of three batches were measured according SOP just after taking from -20℃ and thawing in room temperature. Repeated 10 times. Calculate residual rate of activity compared with initial one and then draw a stability curve.


CONCLUSION:


RCPB solution (10mg/ml) is stable when kept under -20℃ in 1 year.

RCPB solution (10mg/ml) is stable during 10 cycles repeated freezing and thawing.


Fig 1. Long term stability of RCPB solution (10mg/ml) for 12 months kept under -20℃


Fig 2. Stability of RCPB solution (10mg/ml) during 10 cycles repeated freezing and thawing



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ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u Images

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